- the enzyme LCHAD is part of the mitochondrial trifunctional protein complex consisting of 3 enzymes:
Long-chain 3 ketoacyl- CoA thiolase
Long-chain 3 hydroxyacyl- CoA dehydrogenase (LCHAD)
Long-chain enoyl- CoA hydratase
- Differentiation from TFP- deficiency is possible on the basis of a genetic examination
- Mutation analysis in the HADHA (LCHAD -a) gene (with a uniform mutation in the majority of all Central European patients) or HADHB (LCHAD-ß) gene
- Findings from the screening lab:
increased concentration of long-chain hydroxylated acetylcarnitines (C14OH, C16OH, C18:1OH)
- the examination of the organic acids in the urine and the typical acylcarnitine profile in the blood allows a suspected diagnosis, but cannot distinguish LCHAD deficiency from MTP deficiency
- the diagnosis can only be confirmed by detection of the G1528C mutation or by determination of the enzyme activity
- most frequent mutation G1528C (90% of mutant alleles)
(Source: http://www.orpha.net )